Analysis of free radicals in biological systems

"The main aim of the book is to provide a comprehensive survey on recent methodological aspects of the measurement of damage within cellular targets, information which may be used as an indicator of oxidative stress."--BOOK JACKET. "In the introductory chapters, emphasis is placed on the chemical properties of reactive oxygen species and their role in the induction of cellular modifications together with their links to various diseases. The central part of the book is devoted to the description of selected methods aimed at monitoring the production of free radicals in cellular systems. In addition, several assays are provided to assess the chemical damage induced by reactive oxygen species in critical cellular-targets in vitro and in humans in vivo."--BOOK JACKET. "Thus both practical aspects and general considerations, including discussions on the applications and limitations of the assays, are critically reviewed. One of the major features of the book is the description of new experimental methods. These include the measurement of oxydized DNA bases and nucleosides, new techniques for the determination of LDL oxidation using spin-trap agents, and the use of salicylate as an indic